Create a high resolution figure for chain

For both PyMOL and Chimera, download the modified PDB file showing insufficient data (or the PDB file hiding insufficient data), which contains ConSurf's color grades.
Please note: We recommend creating a directory where all files will be saved.

For PyMOL:
1. Download the coloring script.
2. Start PyMOL program. Please note: when using PyMOL commands, all files should be typed with the absolute path (their location on your computer).
3. Load the modified PDB file with the PyMOL viewer window, by typing: PyMOL>"load modified_pdb_full_path" (without the quotes) and hitting return.
4. Run the coloring script with the PyMOL viewer window, by typing: PyMOL>"run coloring_script_full_path" (without the quotes) and hitting return.
5. Save the high quality figure with the PyMOL viewer window, by typing: PyMOL>"ray height,width" (without the quotes) and hitting return, and then by typing PyMOL>"png filename.png" (without the quotes) and hitting return.

For ChimeraX:
1. Download the coloring script.
2. Start ChimeraX program.
3. Load the modified PDB file using the File/Open menu.
4. Load the coloring script using the File/Open menu.
5. Save the high quality figure using the File/Save Image menu.